IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

mung bean nuclease

Mibi ifai at po.uni-stuttgart.de
Fri Dec 15 06:42:48 EST 1995


You may be getting some correct digestion, but it may be swamped out by
a background of intact EcoRI ends that are favored in the ligation step.
Try recutting the mix with EcoRI after the mung bean step, before you 
transform.



More information about the Methods mailing list

Send comments to us at biosci-help [At] net.bio.net