IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

Phage Display for DNA binding proteins?

Peter Wang plw at mrc-lmb.cam.ac.uk
Sun Dec 17 13:39:44 EST 1995

pnh at cockleberry.ncifcrf.gov (Paul N Hengen) wrote:
>I'm considering using a phage display system and I was wondering if anyone has
>used such a library to pan out specific DNA binding proteins or peptides by
>using an immobilized DNA? ...<SNIP>

Quite a bit of work has been done on phage display of zinc-fingers to create novel sequence-specific DNA binding proteins:

REVIEW:  Choo Y, Klug A. Curr. Opin. Biotechnol. (1995) 6:431
Rebar EJ, Pabo CO. Science (1994) 263:671
Jamieson AC, Kim SH, Wells JA.  Biochemistry (1994) 33:5689
Choo Y, Klug A.  PNAS (1994) 91:11163 and 11168
Wu H, Yang W, Barbas CF.  PNAS (1995) 92:344

As discussed in the review, a potential problem for other classes of DNA binding proteins is that they often bind DNA as dimers; so if you use a "monovalent" phage display system or if the protein cannot form dimers when displayed multivalently on phage, this approach may not work.

Peter Wang, M.D., Ph.D.
MRC Centre for Protein Engineering,
Hills Road, Cambridge, CB2 2QH, England

Tel (01223) 402100  (international calls +44-1223-402100)
    (01223) 402104  (direct number)
Fax (01223) 402140

More information about the Methods mailing list

Send comments to us at biosci-help [At] net.bio.net