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Phage Display for DNA binding proteins?

Peter Wang plw at mrc-lmb.cam.ac.uk
Sun Dec 17 13:39:44 EST 1995


pnh at cockleberry.ncifcrf.gov (Paul N Hengen) wrote:
>I'm considering using a phage display system and I was wondering if anyone has
>used such a library to pan out specific DNA binding proteins or peptides by
>using an immobilized DNA? ...<SNIP>

Paul:
Quite a bit of work has been done on phage display of zinc-fingers to create novel sequence-specific DNA binding proteins:

REVIEW:  Choo Y, Klug A. Curr. Opin. Biotechnol. (1995) 6:431
ORIGINAL REFS:
Rebar EJ, Pabo CO. Science (1994) 263:671
Jamieson AC, Kim SH, Wells JA.  Biochemistry (1994) 33:5689
Choo Y, Klug A.  PNAS (1994) 91:11163 and 11168
Wu H, Yang W, Barbas CF.  PNAS (1995) 92:344

As discussed in the review, a potential problem for other classes of DNA binding proteins is that they often bind DNA as dimers; so if you use a "monovalent" phage display system or if the protein cannot form dimers when displayed multivalently on phage, this approach may not work.

---------------------------------------------------------
Peter Wang, M.D., Ph.D.
MRC Centre for Protein Engineering,
Hills Road, Cambridge, CB2 2QH, England

Tel (01223) 402100  (international calls +44-1223-402100)
    (01223) 402104  (direct number)
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