His-tag fusion protein vector

Eric C. Anderson e-anderson at ski.mskcc.org
Tue Dec 12 17:50:53 EST 1995


In article <4ahq3j$ld7 at news.duke.edu>, Namjin Chung <chung006 at mc.duke.edu>
wrote:

> Dear Netters,
> I need mammalian expression vector with His-tag at C-terminus, but I
only could find one 
> with His at N-terminus from Invitrogen.  Does anyone know if C-terminal
His-tag fusion 
> protein vector is available?  I'm afraid if I should make my own
expression vector.  If so, is 
> there any suggestions for space between the protein of my interest and
His(6), and for the 
> amino acid composition of the space peptide.  Thanks in advance, and
please forward your 
> comments to chung006 at mc.duke.edu.

Qiagen also makes N and C terminal His-tag vectors.

eric

-- 
Eric C. Anderson
Cell Biology and Genetics
Memorial Sloan-Kettering Cancer Center
Sloan-Kettering Institute
1275 York Ave., Box 470
New York, NY  10028
Phone: (212)639-2977
Fax:   (212)717-3298
e-anderson at ski.mskcc.org



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