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fungal RNA

Robyn Lee hrarhl at marc.cri.nz
Mon Dec 18 20:02:40 EST 1995


Hi everyone. I'm having problems with RNA extracted from the fungus 
Glomerella cingulata. I use TRIzol and have tried extra phenol:chloroforms 
and ethanol precipitations etc. but can not get any first strand synthesis 
to occur. Does anybody have any ideas on whats going on and how I can 
overcome this problem? 
Thanks for any help, and Merry Christmas.
Robyn.



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