Software to read SDS-PAGE
horton at biosci.cbs.umn.edu
Mon Dec 18 14:11:33 EST 1995
Dima Klenchin (klenchin at macc.wisc.edu) wrote:
: In article <dtyyu.148.0013AA4D at ucla.edu>,
: dtyyu at ucla.edu (David Tak Yan Yu) wrote:
: >Is there a free software to calculate MW of proteins in SDS-PAGE?
: I don't get it. Do you need imaging program to be able to scan
: you gel and then digitize/edit it or you need to calculate MW?
: The latter does not require software: plot log(MW) vs distance
: and from this calibration curve (ideally,line) calculate
: MW of you protein. There are many caveats discussed in many
: sources but software just can't help here.
There are actually software programs that will fond the bands in an
image file, measure how far they migrated, and calculate MW
One program for the Macintosh is called "GelReader" from NCSA. One
little hint; when we tried it, it required that you have standards at
both ends of the gel. This was not obvious from the documentation at the
time (I don't know if they've clarified that point yet).
You can download it from:
The file you choose depends on whether you have a floting point
co-processor or not.
This actually links you to
(I put in the ftp address in case anybody has ftp access but not www. If
you're not familiar with this "URL" format for an ftp address, it means
you use ftp to the directory /Mac/GelReader/ of the server
Please let me know if you actually use the ftp address, rather than the
www site. If no one finds this useful, I won't bother to give both ways
in the future.
Here's more info copied from their web page:
===== Stuff from the NCSA GelReader for Macintosh Page follows: =====
NCSA GelReader is a color-imaging and analysis tool developed for
molecular biologists or others in need of electrophoretic gel
data analysis. The program extracts information from digitized
electrophoretic gel images such as DNA fragments. Allowing
interactive manipulation of lanes and bands, the tool helps
estimate the molecular weight of bands as well as generate
reports. NCSA GelReader was created with the assistance of UIUC's
Center for Prokaryotic Genome Analysis.
NCSA GelReader for the Macintosh: Overview
NCSA GelReader 2.0 is a software tool developed to help scientists
extract information from digitized electrophoretic gel images. It was
specifically designed for molecular biologists doing large-scale,
physical mapping, but its usefulness extends to other scientists
performing DNA, RNA and protein research as well.
Specifically, NCSA GelReader 2.0 employs the color-imaging capabilities
of the Macintosh to help the molecular biologist estimate the molecular
weights of electrophoretically separated DNA fragments.
NCSA GelReader 2.0 allows you to:
find the lanes in a gel image, semi-interactively
add and delete lanes interactively
find the bands (sites) in the image automatically add and delete
adjust the sensitivity of the band and lane searching algorithms
create and modify standards that you can save as part of the program
for subsequent use or use to assign standards to lanes and then
create assay templates that you can save and reuse. These templates
define the lane names, specify some lanes as standards, and
designate what those standards are.
generate reports of the molecular weights of the unknowns or their
load gel images from TIFF, PICT, HDF or raw binary files
save the results of the analysis into an HDF file along with the gel
view the profiles of the lanes in the same window with the gel
superimpose standard lanes and bands copied from one image onto
another using registration marks to identify the geometric
relationship between the two images. This feature is useful if you
are multiplexing Southern blots.
To use NCSA GelReader 2.0 you need a Macintosh with 256 color or
gray-scale capabilities, at least two megabytes of RAM and a monitor.
Additionaly, you will need to digitize the gels. The Hewlett Packard
ScanJet Plus 8-bit scanner is one mechanism that will allow you to do
this. Whatever the vehicle you use to digitize the gel, the gel image
file will have to be in a format (TIFF, PICT, HDF or raw binary files)
that the GelReader will be able to read.
======= Here Endeth the Stuff from the NCSA GelReader Page =======
Robert M. Horton (PhD!) *CAUTION: .sig under construction*
U of M Dermatology Dept.
Box 98 UMHC, 4-154 PWB (612)625-8941 http://220.127.116.11
Minneapolis, MN 55455 horton at biosci.cbs.umn.edu Disclaimer:"Bob who?"
More information about the Methods