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Expert advice needed on mutations/deletions

Yong Shu Liu liu at ariel.its.unimelb.edu.au
Mon Dec 18 23:00:58 EST 1995


Dear all,
	I have found a deletion in an oncogene. The deletion occured at where
people normally found mutation (G>A or G>T etc.). The deletion caused an open
reading frame shift and resulted a stop codon straight away. Therefore the
protein was truncated (only 14 AA residues). The deletion was found in a
tumour and not in the adjacent tissue. My question is: Is there any way I can
relatively easily find out the deletion is in just one of the two alleles or
in both? This deletion is obviously tumour related. However, if an oncogene
is knocked-out in a tumour, then whether that gene is an oncogene becomes
question. Am I right and making sense? 
	Any advice and discussion are extremely welcomed.
	In the natural circumstances, one allele of the two becomes active and
the other remains inactive. If there is anything wrong with the active one,
the other then switch on. Is that right?
	Your suggestions are absolutely needed.
	Thank you very much in advance.

-- 
Yong Shu LIU,      Ph.D. | **.           .***.   .***.           .***.   .**
Department of Surgery    | | | *       * | | | * | | | *       * | | | * | |
University of Melbourne  | | | | *   * | | | *   * | | | *   * | | | *   * |
Western Hospital         | * | | | * | | | *       * | | | * | | | *       *
Footscray                |   '***'   '***'           '***'   '***'
Victoria 3011            |
Australia                |    |
(61 3) 9319 6779 (O)     |    |      __)         	===================
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(61 3) 9317 8525 (FAX)   |    |        |   |    | 	it wouldn't be fun.
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