phenol/chloroform

Colin Rasmussen ras-1 at bones.biochem.ualberta.ca
Tue Dec 19 11:38:48 EST 1995


> Just out ofcuriosity, when one prepares phenol/chloroform, what is the 
> top phase and why should we not use it?
> 
Because it is the aqueous layer that is formed when the chloroform is 
added to the buffer saturated phenol.  It isn't phenol is why you don't 
use it.

Colin
> 



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