Tom Thatcher ttha at
Tue Dec 19 09:09:09 EST 1995

In <4b60p6$eu5 at> ez022056 at (Edward Wang) writes:

>Just out ofcuriosity, when one prepares phenol/chloroform, what is the 
>top phase and why should we not use it?

If you are mixing water-saturated, Tris-equilibrated phenol with 
chloroform, the top phase is water.  You shouldn't use it because there
is no phenol/chloroform in it :)

Tom Thatcher                          | You can give a PC to a Homo habilis,
University of Rochester Cancer Center | and he'll use it, but he'll use it
ttha at           | to crack nuts.

More information about the Methods mailing list