phenol/chloroform

[Tom Thatcher]

In <4b60p6$eu5 at mark.ucdavis.edu> ez022056 at bullwinkle.ucdavis.edu (Edward Wang) writes:

>Just out ofcuriosity, when one prepares phenol/chloroform, what is the 
>top phase and why should we not use it?

If you are mixing water-saturated, Tris-equilibrated phenol with 
chloroform, the top phase is water.  You shouldn't use it because there
is no phenol/chloroform in it :)


-- 
Tom Thatcher                          | You can give a PC to a Homo habilis,
University of Rochester Cancer Center | and he'll use it, but he'll use it
ttha at uhura.cc.rochester.edu           | to crack nuts.