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electophoresis of total-DNA(cut)

Hiranya Roychowdhury hroychow at NMSU.EDU
Tue Dec 19 15:35:56 EST 1995

On 19 Dec 1995, Petur Henry Petersen wrote:

> 	I was cutting total-DNA with Hind III and got a smear
> on a 0.8% Agarose gel. Is this the way it should be or is
> one supposed to see any bands?
> php at iti.is
> Marine Research Institue of Iceland
Yes, you should see a smear on the gel if you are talking about total 
GENOMIC DNA. This is because the RE generates almost all possible sizes 
of fragments from the genomic DNA... sometimes, if you look carefully, 
you should be able to see a few relatively more intense bands in the 
smear. These are probably fragments generated from some moderate 
to highly repetitive sequences. 

			  Hiranya S. Roychowdhury
   			  Plant Genetic Engineering Lab.
			  Box 3GL, NM State Univ.
			  Las Cruces, NM 88003
			  Phone: (505) 646-5785
			  hroychow at nmsu.edu

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