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How to elute antigens without eluting Ab off Prot. A?

Giorgio Spagnol spagnol at galactica.it
Wed Dec 20 03:25:28 EST 1995


You may try omitting the reducing agent from the loading buffer.
This way the Ab should remain in the top part of the gel. I would also 
try reducing the concentration of the Ab.
Hope it helps.
Giorgio





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