spurious large products seen following RT-PCR

drodenhi at julian.uwo.ca drodenhi at julian.uwo.ca
Wed Dec 20 16:40:43 EST 1995


When performing RT-PCR, has anyone observed a continuous smear of spurious
products all of which are larger than the predicted PCR product, which is
also still present? (The spurious bands form a continuous "smear" above
the expected band.) The smear can also be defined as discrete bands as the
PCR conditions are optimized. Thanks for any explanations and/or ways to
eliminate this problem.



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