Transposon mutagenesis of Vibrios

Paul N Hengen pnh at cockleberry.ncifcrf.gov
Wed Dec 20 13:08:47 EST 1995


Ron Caspi (rcaspi at sdcc3.ucsd.edu) wrote:

: I am posting this for a friend who wants to use transposon mutagenesis
: with marine Vibrios. Does anybody have experience with that (vectors,
: selection markers, etc.). I have a system that I'm using with a
: Pseudomonas, but it doesn't seem to work that well with the Vibrio.
: Any help will be highly appreciated!

Ron:

Rita Colwell at the University of Maryland has used the suicide vector pGS9
successfully with Vibrio. pGS9 is the transfer operon of IncN plasmid pCU1
cloned into pACYC184 carrying transposon Tn5.

@article{Selvaraj.Iyer1983,
author = "G. Selvaraj
     and V. N. Iyer",
title = "Suicide plasmid vehicles for insertion mutagenesis in
Rhizobium meliloti and related bacteria",
journal = "J. Bacteriol.",
volume = "156",
pages = "1292-1300",
year = "1983"}

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