biotin ddNTP

Theo Nikiforov nikifor at cts.com
Wed Dec 20 21:50:47 EST 1995


You can buy biotinylated, fluoresceinated, or amino-modified ddNTPs from 
DuPont NEN (Wilmington, DE). You can use the amino groups on the latter 
to attach whatever label or hapten you want. Instead of 
streptavidin-alkaline phosphatase, I recommend using 
anti-biotin-alkaline phosphatase, or horseradish peroxydase,  which will 
give you better sensitivity. Check the catalogs of Biodesign, Vector, 
Zymed and Boehringer. One reference that might be useful: Nucleic Acids 
Research, 1994, Vol. 22, 4167-4175. good luck. 





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