Pac I restriction enzyme

Calydonian Calydon at best.com
Wed Dec 20 20:32:38 EST 1995


     I am trying to clone a gene into a plasmid using a unique PacI site.
PacI leaves an AT overhange and I suspect this could be causing my
problems. Any suggestions on what ligation conditions to use (ie ligation
at 10C) would be greatly appreciated!!

Thank you,
Andrew Little



More information about the Methods mailing list