Pac I restriction enzyme

Calydonian Calydon at
Wed Dec 20 20:32:38 EST 1995

     I am trying to clone a gene into a plasmid using a unique PacI site.
PacI leaves an AT overhange and I suspect this could be causing my
problems. Any suggestions on what ligation conditions to use (ie ligation
at 10C) would be greatly appreciated!!

Thank you,
Andrew Little

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