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pyronin y----acid buffer dye front

Wed Dec 20 21:06:05 EST 1995

You can add 5 ul of pyronin Y (0.05% solution, in 50% glycerol) per 
lane.  It will migrate just ahead of where the bromophenol blue dye 
front normally migrates.  (This protocol is from Immunetics, and we 
have used it.)
Glenn Cantor
Dept Vet Micro Path
Washington State University

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Date sent:      Sat, 16 Dec 1995 19:41:08 -0800
From:           Glenn Cantor <gcantor at vetmed.wsu.edu>
To:             gcantor at vetmed.wsu.edu
Subject:        pyronin y----acid buffer dye front

>From BIOSCI-REQUEST  Mon Nov 27 13:22:42 1995
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>From: davesmith at bioch.tamu.edu
Subject: pyronin y----acid buffer dye front
Date: Mon, 27 Nov 1995 23:02:55 GMT
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We're gearing up to do native PAGE on a basic protein and need a dye
front/ marker.  We've heard of others using pyronin y, but don't have
a protocol.   Can anyone help us out?


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