pyronin y----acid buffer dye front

Glenn Cantor GCANTOR at VETMED.WSU.EDU
Wed Dec 20 21:06:05 EST 1995


You can add 5 ul of pyronin Y (0.05% solution, in 50% glycerol) per 
lane.  It will migrate just ahead of where the bromophenol blue dye 
front normally migrates.  (This protocol is from Immunetics, and we 
have used it.)
Glenn Cantor
Dept Vet Micro Path
Washington State University

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From:           Glenn Cantor <gcantor at vetmed.wsu.edu>
To:             gcantor at vetmed.wsu.edu
Subject:        pyronin y----acid buffer dye front

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>From: davesmith at bioch.tamu.edu
Subject: pyronin y----acid buffer dye front
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We're gearing up to do native PAGE on a basic protein and need a dye
front/ marker.  We've heard of others using pyronin y, but don't have
a protocol.   Can anyone help us out?
Thanks,
Dave




 




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