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Ultracompetent E.coli cells!?! - A New Summary

David Ish-Horowicz d_horowicz at icrf.icnet.uk
Thu Dec 21 04:50:48 EST 1995


In article <4arlrj$nbe at elna.ethz.ch>, Alexander Kraev
<kraev at bc.biol.ethz.ch> wrote:

[stuff deleted]

>  There is no mention of the water quality in Inoue et al. Is it by default 
> very good (everybody has a MilliQ machine, right?) or it does not really 
> matter? 

It matters. In Oxford, whose water quality is terrible, we found we
couldn't make decent (>=10^7) competent cells until we bought (!) AR water
(originally for other reasons). Our Milli-Q'd water didn't work.

David Ish-Horowicz
Imperial Cancer Research Fund, London, England
horowicz at icrf.icnet.uk



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