Cloning with large plasmids

Mikhail Alexeyev malexeyev at biost1.thi.tmc.edu
Thu Dec 21 12:49:23 EST 1995


In article <Calydon-201295182443 at calydon.vip.best.com>, Calydon at best.com
(Calydonian) wrote:

>      I am trying to clone a gene into a 37kb plasmid that has a tendancy to
> rearrange. Do you have any suggestions on how to stop this rearrangement
> from happening. 
> 

Gibco sells Stable2 E. coli strain. According to company claim, it should
help. I worked with strain, but didn't use it to propagate unstable
iserts, so this is not from experience.

M. Alexeyev



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