Cloning with large plasmids

Mikhail Alexeyev malexeyev at
Thu Dec 21 12:49:23 EST 1995

In article <Calydon-201295182443 at>, Calydon at
(Calydonian) wrote:

>      I am trying to clone a gene into a 37kb plasmid that has a tendancy to
> rearrange. Do you have any suggestions on how to stop this rearrangement
> from happening. 

Gibco sells Stable2 E. coli strain. According to company claim, it should
help. I worked with strain, but didn't use it to propagate unstable
iserts, so this is not from experience.

M. Alexeyev

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