Estimating copy number in transgenic mice

T.J.Anderson gvsa07 at UDCF.GLA.AC.UK
Thu Dec 21 10:35:45 EST 1995


I would like to see what the opinion is on the best techniques
available  to distinguish between hemizygous and homozygous mice with
with low (3-7) copies of a transgene that contains a wild type
gene. How confident would people be that they can distinguish
between hemizygous and homozygous animals.
The current probe hybridises to both transgene and wild type 
DNA sequences.

As a supplementry question I wonder what degree of confidence
one might be able to count the extra numbers of the gene 
contributed by the transgene and what is considered to be
the "gold standard" technique for doing this.





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