Cloning with large plasmids

Scott Earley scottearley at
Thu Dec 21 22:44:49 EST 1995

Need More Detail, e.g. what plasmid, what gene, etc. You might try a
different host strain.

In article <Calydon-201295182443 at>, Calydon at
(Calydonian) wrote:

>      I am trying to clone a gene into a 37kb plasmid that has a tendancy to
> rearrange. Do you have any suggestions on how to stop this rearrangement
> from happening. 
> Thank you,
> Andrew Little

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