help with DNA sequencing gels
szjmahr at peseta.ucdavis.edu
Fri Dec 22 11:41:53 EST 1995
Hello there - i am having problems with sequencing gels that are
creeping up between the plates in a voltage dependent manner (if i leave
the gel running it slips out further than if I leave it off). I am using
FisherBrand Urea and Acrylamide. I coat one plate (always the same one)
with sigmacote after scrubbing the hell out of them with a green pad and
detergent and soap. The plates are from Hoeffer and it is only with
these plates that I have them problem. I run my gels at a constant 40Watts.
The creeping always begins right away as soon as I begin to prerun the gel.
Needless to say this is a veery frustrating experience. Please Help!
sincerly - jamahr at ucdavis.edu
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