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35S or 33P?

Peter Fear fearp at vax.cs.hscsyr.edu
Fri Dec 22 14:08:34 EST 1995

    A researcher here showed us a document from Perkin Elmer which states
"There have been reported occurances of alpha-35S label escaping from
various types of reaction tubes and contaminating thermal cycler blocks. 
We strongly recommend the use of [alpha-33P]-dNTP for cycle sequencing."

Our question is not if there is any 35S given off but rather what is the
level and if this is really a problem if done in a hood?

Probably any answers should be sent directly to me at fearp at vax.cs.hscsyr.edu



Peter Fear
Radiation Safety Office
SUNY Health Science Center
750 E. Adams St.
Syracuse, NY 13210
fearp at vax.cs.hscsyr.edu

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