> Dr Patrick HJ Falckh <p.falckh at rmit.edu.au> writes:
>> In article <4ad9fc$km2 at dub-news-svc-2.compuserve.com>,
>100414.220 at compuserve.com (Stefan Kley) says:
> >Hi there,
> >I have a problem with ethidium bromide. I ran a PAGE today to check
> >the integrity of a few cDNA probes that I'm about to use in Northern
> >Hybridisations. In order to visualize the DNA with UV light, I put the
> >gel into an ethidium bromide bath for 45 minutes after the run.
> >However, under UV light, the gel appeared empty, lacking a signal even
> >from the slot where I loaded the DNA standard.
>> I also add EtBr into the gel (agarose) but not into the buffer. The
> advantage of this is that the amount of EtBr you use, and then liable to
> spill, is limited. The EtBr moves in the opposite direction to your
> cDNA and thereby decreases background. Using agarose is also much
> Patrick Falckh
>>>>>>>>Use SYBR Green. It is much better, safer, and does not give the background problems commonly associated with ethidium bromide.
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