the effect of tRNA on cDNA cloning ?
smori at nmsu.edu
Wed Dec 13 21:05:22 EST 1995
Seongwook Kwon (swkwon at email.unc.edu) wrote:
: I am thinking of using tRNA to help out precipitating my cDNAs. These
: cDNAs are to be used for a library construction.
: Does anyone know the effect of tRNA presence on 1) conversion of ss
: cDNA into ds cDNA (I will use Klenow), 2) ligation of insert DNA into
: lambda vector 3) packaging of vector-insert into lambda particles ?
You can do two things if tRNA's bother you;
1) Use 10mg/ml glycogen (add 5ul to your DNA) and then add NaOAC and 95%
2) Add a little Rnase to the resuspended pellet.
C A N A D A
Program in Molecular Biology
Department of Chemistry and Biochemistry Box 3C
New Mexico State University
Las Cruces NM
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