as at macpost.pharmakologie.uni-erlangen.de
Wed Dec 27 04:48:36 EST 1995
In article <4ank80$hgc at moonbeam.aecom.yu.edu>, auster at alsys1.aecom.yu.edu
> I eluted protein from an affinity column with 1% SDS (various salt
> concentrations did not work), and now I need to remove the SDS from the
> protein. Does anybody know of a good method for doing so? Please
> e-mail me directly at: auster at aecom.yu.edu. Thank you very much!
I know of two methods which I have used successfully to purify proteins
that I had eluted from SDS-gels. Both resulted in sufficiently pure
protein for direct N-terminal sequencing.The references are:
Wessel, D. & Flügge, U.I. (1984) A Method for the Quantitative Recovery of
Protein in Dilute Solution in the Presence of Detergents and Lipids. Anal.
(in case the name of the second author is partly garbage - the third
letter is a german umlaut U)
Konigsberg, W.H. & Henderson, L. (1983) Removal of Sodium Dodecyl Sulfate
from Proteins by Ion-Pair Extraction. Meth. Enzymol. 91:254-259.
Axel Schumacher, Ph.D. ! Phone:+49 9131 852002
Institute for Pharmacology ! FAX: +49 9131 206119
University of Erlangen !
D-91054 Erlangen Germany ! as at macpost.pharmakologie.uni-erlangen.de
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