In article <4bqt6k$8kr at dingo.cc.uq.oz.au>, forrest at biosci.uq.oz.au} says...
> Since then I've used an ultracentrifuge with eppendorf appadters
>to purify fragments in the range of 100bp and above for cloning and this
>seems to be pretty cheap too.
>>TLA100 60,000 4C for 15 minutes, take supernatant and etoh ppt. This will
>probably be OK for automated sequencing. I don't know where the protocol
>came from originally just ppl in the lab were using it and it works for
> Alistair Forrest (PhD student)
How specifically are you smashing up your frozen agarose chip?