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HELP! How do you purify a 150bp PCR product for sequencing

Harry Harry.Witchel at bristol.ac.uk
Thu Dec 28 08:15:13 EST 1995


In article <4bqt6k$8kr at dingo.cc.uq.oz.au>, forrest at biosci.uq.oz.au} says...
>
>

>      Since then I've used an ultracentrifuge with eppendorf appadters
>to purify fragments in the range of 100bp and above for cloning and this
>seems to be pretty cheap too.
>
>TLA100 60,000 4C for 15 minutes, take supernatant and etoh ppt. This will
>probably be OK for automated sequencing. I don't know where the protocol
>came from originally just ppl in the lab were using it and it works for
>cloning.
>
>Good luck,
>   Alistair Forrest (PhD student)


Alistair --
	How specifically are you smashing up your frozen agarose chip?
Thanks,
Harry




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