Over the years I have seen a lot of good (and some silly) questions
relating to the biochemistry of the polymerase chain reaction. Many are
answered clearly in the FAQ (kudos to the maintainer(s)), but I am now
looking for a reference which I used to have but can no longer obtain.
Could someone please post the reference (or even the article, publishers
willing) to this newsgroup as a service to the reader?
The article in question was by Kary Mullis and appeared (sometime in the
past 7 years) in "PCR" or "Methods: a Companion to Methods in
Enzymology." The title described "...How to avoid Cold
Oligodeoxyribonuclear Fusion..." Not many libraries get this journal,
and I could not obtain it even last year.
N.B.- I have a small bet (I wish it were a large bet) that dNTP's are
not limiting in normal PCR. I will do the simple calculation myself, but
would still like the reference 8-<)>
For my part, I have attached to this message another excellent reference
describing the basic biochemistry of PCR.
Thank you for your assistance!
Dr. David J. Meyer
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Search request: F PA BLOCH # AND TW POLYMERASE
Search result: 5 citations in the Medline database
Type EXPLAIN LOCATE for library locations; HELP for other display options.
UI - 91175712
AU - Bloch W
AD - Cetus Corporation, Emeryville, California 94608.
TI - A biochemical perspective of the polymerase chain reaction.
TA - Biochemistry
DP - 1991 Mar 19
VI - 30
IP - 11
PG - 2735-47
JC - A0G
IS - 0006-2960
LA - Eng
MH - Base Sequence
MH - DNA/GENETICS
MH - Genes
MH - Mutagenesis
MH - Polymerase Chain Reaction/METHODS
MH - Proteins/GENETICS
RN - 9007-49-2 (DNA)
PT - Journal Article
PT - Review
PT - Review, Academic
RF - REVIEW ARTICLE: 185 REFS.
SB - M
SO - Biochemistry 1991 Mar 19;30(11):2735-47