Well, you know you have the 3' end if you find the poly A tail....
only way to verify 5'end is to perform a type of experiment such as
RACE, primer extension, S1 nuclease assay, or a rnase protection assay.
I would recommend RACE as a starter....then primer extension or RNAse
Xin-Hua Zhou (xz at KEPLER.UNH.EDU) wrote:
: Fi, Netter:
: I am sequencing the 3' end of a cDNA clone. How can I know it contains a
: full length DNA in addition to looking at STOP codens? Any ideas or
: Thank you very much!!
: Xinhua Zhou
..... Martin Leach Email:leach at bu.edu
_|____ Dept. of Pharmacology Phone: (617) 638-5323
/ o / Boston Univ. School of Med. Fax: (617) 638-4329
_/ |-/__==/ 80 E. Concord St. (L603)
(BULLDOZER) \_ Boston MA 02118 "Not the old underpants
USA on your
My homepage: http://188.8.131.52/Leach.html
Biotech company WWW REgistry has moved to: http://www.data-transport.com
Check out the Biotech Rumor mill: http://184.108.40.206/jumper/rumor.html