Does a cDNA clone contain a full length????????
leach at bu.edu
Fri Dec 29 12:06:01 EST 1995
Well, you know you have the 3' end if you find the poly A tail....
only way to verify 5'end is to perform a type of experiment such as
RACE, primer extension, S1 nuclease assay, or a rnase protection assay.
I would recommend RACE as a starter....then primer extension or RNAse
Xin-Hua Zhou (xz at KEPLER.UNH.EDU) wrote:
: Fi, Netter:
: I am sequencing the 3' end of a cDNA clone. How can I know it contains a
: full length DNA in addition to looking at STOP codens? Any ideas or
: Thank you very much!!
: Xinhua Zhou
..... Martin Leach Email:leach at bu.edu
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