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long pcr

Jose M. Bautista jbautist at hgmp.mrc.ac.uk
Fri Dec 29 12:05:49 EST 1995

On 4 Dec 1995, Benke/Belmar Login wrote:

> Could somebody offer me information about 
> long pcr? Which companies sell systems
> devised for such a purpose? What is the
> maximum size of target DNA which can
> be amplified using this procedure?
> Thanks in advance.
> Sincerely,
> Tammy J. Rossi
> Res. Clin. Spec.
> Univ. Miami Sch. Med.
> benke2 at peds.miami.edu
I have been using susccessfully Stratagene and Boehringer kits. The 
latter somehow much better. I have been able to amplify up to 10kb. 
Sometimes it is required to carry out a second PCR round with 1 ul of the 
first reaction.

Hope it help

Jose M. Bautista

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