I have been doing pulse chase experiments with radiolabelled amino
acids followed by immunoprecipitations in order to detrmine the half
life of the protein I work with. I was wondering what the best way is
to determine how much labelled protein to add to the protein A
sepharose beads. The two methods I have been using are counts by TCA
precipitation and total protein determination. Can someone suggest a
better way or tell me which way is preferable.