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Problems with Autofluorescence?

Kevin Henne khenne at fred.fhcrc.org
Sat Dec 30 16:06:38 EST 1995


I've tried quenching fluorescence caused by formalin fixation in 
paraffin embedded tissue with 0.14mg/ml crystal violet and also by a 
longer treatment in 0.1M glycine. Neither method seems to decrease 
background levels so that it doesn't mask/overshadow FITC or Texas 
Red. I'm limited to using tissue fixed and processed in this manner.
Thanks for your suggestions,
Kevin Henne (khenne at fred.fhcrc.org)



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