PCR and polymerase.

Mibi ifai at po.uni-stuttgart.de
Sun Dec 31 10:43:35 EST 1995


In article <30DB28E4.2184 at icbr.ifas.ufl.edu>, "bruce w. ritchings" <bwr at icbr.ifas.ufl.edu> says:
>
>Dale Levitzke wrote:
>> 
>> Hello netters,  I am currently using exo-pfu polymerase for the
>> amplification of a G-C rich PCR.  Has anyone had any experiance in using
>> this enzyme?  Has anyone had any experiance in the amplification of G-C
>> rich templates and if so what was your enzyme of choice ?
>> Would greatly appreciate any input.

Have you seen "Use of dITP in PCR to Improve Amplif. of GC-rich DNA"? 
(BioTechniques 19, 48)

Good luck.



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