precipitate in NEB T4 Ligase buffer?

Randy Haun rhaun at
Wed Feb 1 10:41:34 EST 1995

In article <jpcd0-0102951114380001 at> jpcd0 at (John Dixon) writes:
>From: jpcd0 at (John Dixon)
>Subject: precipitate in NEB T4 Ligase buffer?
>Date: Wed, 01 Feb 1995 11:14:38 +0000
>Just a quickie:
>Does anyone know what the white precipitate is that sometimes forms in NEB
>T4 Ligase buffer?

I think it's the DTT. . .

>It seems to redisolve after heating to 37 C and vigorous shaking, but I am
>wondering whether repeated heatings may degrade the ATP or anything else
>in the reaction buffer.
>Cheers,  Johnny D
>John Dixon                     Lab 44 (223) 334131
>Wellcome/CRC Institute         Fax 44 (223) 334134
>Dept Genetics
>Cambridge University    
>United Kingdom       e-m: jpcd0 at

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