precipitate in NEB T4 Ligase buffer?

Randy Haun rhaun at nih.gov
Wed Feb 1 10:41:34 EST 1995


In article <jpcd0-0102951114380001 at macr1-4.welc.cam.ac.uk> jpcd0 at mole.bio.cam.ac.uk (John Dixon) writes:
>From: jpcd0 at mole.bio.cam.ac.uk (John Dixon)
>Subject: precipitate in NEB T4 Ligase buffer?
>Date: Wed, 01 Feb 1995 11:14:38 +0000
>Just a quickie:
>
>Does anyone know what the white precipitate is that sometimes forms in NEB
>T4 Ligase buffer?

I think it's the DTT. . .

>
>It seems to redisolve after heating to 37 C and vigorous shaking, but I am
>wondering whether repeated heatings may degrade the ATP or anything else
>in the reaction buffer.
>
>Cheers,  Johnny D
>
>-- 
>John Dixon                     Lab 44 (223) 334131
>Wellcome/CRC Institute         Fax 44 (223) 334134
>Dept Genetics
>Cambridge University    
>United Kingdom       e-m: jpcd0 at mole.bio.cam.ac.uk



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