nested deletions with no blunt ends?
tpalmer at gpu.srv.ualberta.ca
Thu Feb 2 01:47:14 EST 1995
I need to do 3'nested deletions of an insert in a vector. The only methods
that I have come across for doing this involve the use of exonuclease III
and end up generating blunt ends which are then ligated to recircularize
the vector. Does anyone know of any methods which do not generate blunt
ends? Thanks in advance. Please reply by e-mail:
tpalmer at gpu.srv.ualberta.ca.
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