Temperature to pour plates
aaron at netnews.upenn.edu
Wed Feb 1 22:21:50 EST 1995
WmsFan (wmsfan at aol.com) wrote:
: Here's an easy one for you microbiologists. My son is doing a simple
: science experiment looking at the effect of temperature on growth rates of
: bacteria in milk. We got some sterile agar and plates from a local
: company. We melted the agar, added milk to the plates with sterile
: pipettes, and are about to pour the media....but I imagine we could
: sterilize the milk if we pour the agar too hot. What temperature should we
: cool the agar to before we pour it?
I'd suggest waiting until you touch the agar for about 10 seconds without it
hurting. If you're measuring the temperature, I'd say roughly 50 degrees
centigrade. Also, I wouldn't put an unsterile thermometer into the agar.
Use one of those thermometers you stick on fishtanks.
If you don't have a sterile thermometer or an external thermometer, just go by
touch. Just make sure the agar doesn't start clumping.
More information about the Methods