RNAses

Aaron Pawlyk aaron at netnews.upenn.edu
Wed Feb 1 22:28:18 EST 1995


Kusol Pootanakit (pootanak at hermws.bc.edu) wrote:
: Hi,

: Just out of curiosity, does anyone out there know if after one cross-linked
: RNA on to nylon membrane; can the RNases still get to them.

: thanks

If the RNA is well cross-linked RNases won't have as much of an effect on them
as they will in solution.  The RNA cross-links on its backbone to the membrane
and it will be more difficult for the RNAse to cleave the bonds.  Also, with
the backbone attached the the membrane, it does not make as much of a
difference if the RNA is cleaved; it will still be held into place by the 
cross-linking.

But avoid RNAses anyways.  

			-- Aaron




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