Ethidium Bromide in Samples
nh3 at st-andrews.ac.uk
Wed Feb 1 13:03:24 EST 1995
Dear Julia Lodge,
Adding ethidium bromide directly to samples works well, particularly
when running out RNA using formaldehyde agarose gels prior to Northern
blotting. Try adding 1 microlitre of a 400-1000 microgram/ml stock into each
sample, prior to heating at 65 C or incorporate it directly into your
loading buffer (using the same quantity). The ethidium should even show up
on a filter after
transfer when placed on a transilluminator, so providing a good way of
checking the quality of your blot prior to probing.
Good Luck, Neil Harris.
More information about the Methods