Ethidium Bromide in Samples

Neil Harris nh3 at st-andrews.ac.uk
Wed Feb 1 13:03:24 EST 1995


Dear Julia Lodge,
	Adding ethidium bromide directly to samples works well, particularly 
when running out RNA using formaldehyde agarose gels prior to Northern 
blotting. Try adding 1 microlitre of a 400-1000 microgram/ml stock into each 
sample, prior to heating at 65 C or incorporate it directly into your 
loading buffer (using the same quantity). The ethidium should even show up 
on a filter after 
transfer when placed on a transilluminator, so providing a good way of 
checking the quality of your blot prior to probing.
	Good Luck, Neil Harris.




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