electroporation SP2/0

Mike Preston preston at warren.med.harvard.edu
Wed Feb 1 14:24:29 EST 1995

In article <Pine.SGI.3.91.950125152205.17522B-100000 at helix.nih.gov>,
Richard Braverman <rb at helix.nih.gov> wrote:

>    Anyone out there have any success  in electroporation of vectors into 
> SP2/0...or, alternately, has anyone had success with DOTAP??

We routinely electroporate 10 million SP2/0 cells in regular old PBS with
approximately 10 micrograms of linearized plasmid DNA in 0.4cm cuvettes. We
use a BioRad Gene pulser with a capacitance extender attached.  The voltage
is set at 200 V (0.2kV) and the capacitance set at 960 microfarads. Good

Mike Preston
Channing Laboratory
Brigham and Women's Hospital and Harvard Medical School
180 Longwood Ave. 
Boston, MA 12115
preston at warren.med.harvard.edu

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