precipitate in NEB T4 Ligase buffer?

Colin Rasmussen crasmussen at
Wed Feb 1 20:53:39 EST 1995

In article <D3C9JD.1xM at>, pnh at (Paul N
Hengen) wrote:

> Could it be ficoll? Keeping rATP at 37 C should have no effect on stability,
> else we human types would be in lots of trouble - losing all that energy we
> work so hard to get (?).

It's the DTT. However, repeated freeze-thawing of ATP does have effects,
at least according to one supplier of T4 ligase.

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