precipitate in NEB T4 Ligase buffer?

Colin Rasmussen crasmussen at anat.med.ualberta.ca
Wed Feb 1 20:53:39 EST 1995


In article <D3C9JD.1xM at ncifcrf.gov>, pnh at fcsparc6.ncifcrf.gov (Paul N
Hengen) wrote:


> 
> Could it be ficoll? Keeping rATP at 37 C should have no effect on stability,
> else we human types would be in lots of trouble - losing all that energy we
> work so hard to get (?).
> 

It's the DTT. However, repeated freeze-thawing of ATP does have effects,
at least according to one supplier of T4 ligase.



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