Does Schleicher & Schuell BA-S 85 go off????

HARDIES at THORIN.UTHSCSA.EDU HARDIES at THORIN.UTHSCSA.EDU
Wed Feb 1 14:26:24 EST 1995


jpcd0 at mole.bio.cam.ac.uk (John Dixon) asked if S&S nitrocellulose goes
off and how you can tell if it has.

We see it go off if the role has been opened and kept around a year or 
so.  You can tell if you wet it in water before the transfer buffer.  If
it doesn't wet immediately and uniformly, it's no good.  Typically, the
outer layer of the role and the edges go first and the rest can be salvaged.
So it's obviously exposure to something in the air.  Humidity sounds as
good as any.  I think the problem did get worse when I moved to a more
humid environment, but I can't swear to it.
I assume this would affect nitrocellulose from other vendors
as well, but not nylon based filters.  Do any manufacturers of NC pretreat
their membrane so it stores better?

Cheers,
Steve Hardies, Assoc. Prof. of Biochem., Univ. of Texas HSC at San Antonio
Hardies at uthscsa.edu



More information about the Methods mailing list