precipitate in NEB T4 Ligase buffer?

John Dixon jpcd0 at mole.bio.cam.ac.uk
Wed Feb 1 06:14:38 EST 1995


Just a quickie:

Does anyone know what the white precipitate is that sometimes forms in NEB
T4 Ligase buffer?

It seems to redisolve after heating to 37 C and vigorous shaking, but I am
wondering whether repeated heatings may degrade the ATP or anything else
in the reaction buffer.

Cheers,  Johnny D

-- 
John Dixon                     Lab 44 (223) 334131
Wellcome/CRC Institute         Fax 44 (223) 334134
Dept Genetics
Cambridge University    
United Kingdom       e-m: jpcd0 at mole.bio.cam.ac.uk



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