precipitate in NEB T4 Ligase buffer?
jpcd0 at mole.bio.cam.ac.uk
Wed Feb 1 06:14:38 EST 1995
Just a quickie:
Does anyone know what the white precipitate is that sometimes forms in NEB
T4 Ligase buffer?
It seems to redisolve after heating to 37 C and vigorous shaking, but I am
wondering whether repeated heatings may degrade the ATP or anything else
in the reaction buffer.
Cheers, Johnny D
John Dixon Lab 44 (223) 334131
Wellcome/CRC Institute Fax 44 (223) 334134
United Kingdom e-m: jpcd0 at mole.bio.cam.ac.uk
More information about the Methods