help for labelling probe

[quin chou]

Dear netters:

I am having some problems labelling probes with biotin, either labelling 
DNA template by nick-translation or random priming with biotined dNTPs;
or 5'- or 3'-end labelling through oligo synthesis.  My problem is to get
consistent results with the sensitivity I wanted.  All suggestions or input 
are welcome. Thanks in advance for helps!
Please contact me through above email address.

quinn.