TA Cloning - old product
leach at bu.edu
Thu Feb 2 13:12:33 EST 1995
In article <D3B0Kr.JwG at zoo.toronto.edu>, mes at zoo.toronto.edu (Mark
> I am attempting to clone my pcr product with the TA system. First
> attempt failed.
> Product was about a week old but had been at -20C the whole time.
> Now it's about 2 weeks old though still at -20C.
> Any body know what the likelihood of having lost the adenosine
> residues might be?
I think it should be fine...i've used older products...if in
doubt..incubate the purified product with dATP and Taq pol fro 1-2hrs at
72C in PCR buffer.
..... Martin Leach Email:leach at bu.edu
_|____ Dept. of Pharmacology Phone: (617) 638-5323
/ o / Boston Univ. School of Med. Fax: (617) 638-4329
_/ |-/__==/ 80 E. Concord St. (L603)
(BULLDOZER) \_ Boston MA 02118 "Not the old underpants on your
USA head.....WIBBLE" -BLACKADDER
p.s. try BioMOO (virtual biology on the internet - telnet bioinfo.weizmann.ac.il 8888)
My home page: http://184.108.40.206/Leach.html
More information about the Methods