TA Cloning - old product

[Duncan Clark]

In article: <D3B0Kr.JwG at zoo.toronto.edu>  mes at zoo.toronto.edu (Mark Siddall) writes:
> 
> 
> 
> I am attempting to clone my pcr product with the TA system.  First
> attempt failed.
> Product was about a week old but had been at -20C the whole time.
> Now it's about 2 weeks old though still at -20C.
> Any body know what the likelihood of having lost the adenosine
> residues might be?
> 

Easy solution. Run an extension at 72C for 15mins with just dATP. Single base
should be added. 

Duncan

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