help for probe labelling

[quin chou]

Dear bionetters:

I am having some problems labelling probes with biotin, either labelling
DNA template by nick-translation or random priming with biotined dNTPs;
or 5'- or 3'-end labelling through oligo synthesis.  My problem is to get
consistent results with the sensitivity I wanted.  The probe is used for in situ hybridization. All suggestions or inputs are welcome!

Thanks in advance for helps!

Please contact me through email address: quincicc at netcom.com.

quinn.