Red cell lysis with NH4Cl ?

Steve Konings skonings at post.its.mcw.edu
Fri Feb 3 13:43:08 EST 1995


Mike Morris (mike at medsun.unige.ch) wrote:
: Can someone remind me of the protocol (ie the conc. of NH4Cl needed
: to lyse RBC in human blood)? I haven't done it for years, and I 
: cannot find a recipe anywhere (I guess everyone except me knows
: it by heart :(  

Mike,

I use 0.8% NH4Cl and 0.1% EDTA-Na3 in distilled H20.

Steve Konings
Blood Research Lab
Medical College of WI
Milwaukee - A Great Place On A Great Lake



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