searches and sequence comparisons - how to do?

Tracy Aquilla aquilla at salus.med.uvm.edu
Fri Feb 3 09:01:31 EST 1995


In Article <Pine.3.07.9502021523.B9932-a100000 at wagsun>,
marchaa at agric.nsw.gov.au (A.Marchant) wrote:
>Could someone please tell me how to go about finding and comparing the
>sequences of homologous genes from different organisms, in the sequence
>databases?
>
>What I would like to do is to find published sequences of gene X from
>different organisms, line them up, and identify conserved regions.
>
>Specifically, I am interested in the internal transcribed spacer region of
>ribosomal DNA - a lot of work has been done on this region, from lots of
>different organisms, so perhaps there is a recent published article which
>compares the sequences found from very different taxa.  If anyone
>knows of such (or, better still, has written it!), could they tell me
>about it, please?
>
>Please E-mail replies to me.
>
>        Thanks,  Adam Marchant.
>        marchaa at agric.nsw.gov.au

You need to get the help files for the BLAST and Retreive servers at NCBI.
You may also find NEntrez useful. This information is available via
anonymous FTP. FTP to ncbi.nlm.nih.gov and login as anonymous, using your
email address as a password. Browse through the directories for BLAST and
download the help file. Do the same for Retreive and Entrez (Network). If
you need more help, email me.
    Tracy



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