Cloning in IncN plasmids

Mayaux-bisance mayaux at bisance.citi2.fr
Fri Feb 3 04:38:43 EST 1995


Hello netters,

What i'd like to do is to clone 30 kb by recombination in a PolA
independant vector.
IncN plasmids have an OriS which allow them to replicate in PolA-
Ecoli cells, but I don't know if they are able to carry a 35 kb
fragment.
Has anybody experienced this group of vectors ?
Any information or idea is wellcome.

Laurent.

Email : mayaux at citi2.fr




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