Removing CTAB from Nucleic Acids
dgreen at sbsnov1.auckland.ac.nz
Fri Feb 3 23:36:01 EST 1995
In article <3gquvv$7h6 at ccshst05.cs.uoguelph.ca>, awright at uoguelph.ca
(Andre-Denis G Wright) wrote:
> Dear Netters!
> I extracted DNA using CTAB to remove polyusacchardides, but the CTAB
> coprecipitated with my nucleic acids. I kept the NaCl conc above the
> recommended 0.7M so this wouldn't happen, but it still did. Any suggestions?
Use 1:1 (v/v) chloroform to extract the NaCl resuspended solution. CTAB
moves into the organic phase etc.
School of Biological Sciences
University of Auckland
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