Removing CTAB from Nucleic Acids

David Green dgreen at sbsnov1.auckland.ac.nz
Fri Feb 3 23:36:01 EST 1995


In article <3gquvv$7h6 at ccshst05.cs.uoguelph.ca>, awright at uoguelph.ca
(Andre-Denis G Wright) wrote:

> Dear Netters!
> 
> I extracted DNA using CTAB to remove polyusacchardides, but the CTAB 
> coprecipitated with my nucleic acids. I kept the NaCl conc above the 
> recommended 0.7M so this wouldn't happen, but it still did. Any suggestions?
> 
>                                         Andre

Use 1:1 (v/v) chloroform to extract the NaCl resuspended solution.  CTAB
moves into the organic phase etc.

-- 
David Green
School of Biological Sciences
University of Auckland
New Zealand



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