Temperature to pour plates

Richard_Heath heath at mbcf.stjude.org
Sat Feb 4 19:50:32 EST 1995


In article <3gp7hp$oko at newsbf02.news.aol.com>, wmsfan at aol.com (WmsFan) writes:
> Here's an easy one for you microbiologists. My son is doing a simple
> science experiment looking at the effect of temperature on growth rates of
> bacteria in milk. We got some sterile agar and plates from a local
> company. We melted the agar, added milk to the plates with sterile
> pipettes, and are about to pour the media....but I imagine we could
> sterilize the milk if we pour the agar too hot. What temperature should we
> cool the agar to before we pour it?
> 
> Thanks. 
The agar will set at about 42C.  You thus want to cool to about 45, mix, and
quickly pour the plates.  Short periods at this temperature will not harm most
(if any) of the bacteria.

Good luck with the experiment

Richard




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