Removing CTAB from Nucleic Acids

Michael Cooley szcooley at dale.ucdavis.edu
Mon Feb 6 11:46:09 EST 1995


David Green (dgreen at sbsnov1.auckland.ac.nz) wrote:
: In article <3gquvv$7h6 at ccshst05.cs.uoguelph.ca>, awright at uoguelph.ca
: (Andre-Denis G Wright) wrote:

: > Dear Netters!
: > 
: > I extracted DNA using CTAB to remove polyusacchardides, but the CTAB 
: > coprecipitated with my nucleic acids. I kept the NaCl conc above the 
: > recommended 0.7M so this wouldn't happen, but it still did. Any suggestions?
: > 
: >                                         Andre

: Use 1:1 (v/v) chloroform to extract the NaCl resuspended solution.  CTAB
: moves into the organic phase etc.

: -- 
: David Green
: School of Biological Sciences
: University of Auckland
: New Zealand


temperature is supposed to be a consideration when working with CTAB. If 
the temperature drops below 15 oC the CTAB will precip.


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